AbPure™ BSA Removal Kit

Bovine Serum Albumin (BSA) is often added to purified antibodies as it is an effective stabilizer. However, when labeling antibodies, the BSA becomes a hindrance, as it directly competes with the antibody to attach to the label, greatly reducing the conjugation efficiency. Therefore, prior to undertaking labeling techniques, it is essential to remove the BSA.

Common commercial BSA removal techniques can involve many laborious steps. The AbPure™ BSA Removal Kit effectively separates the BSA from the antibody and can be used on any antibody sub-type or species.

If your antibody is in a complex matrix such as serum or TCS or in presence of gelatin we recommend to use our AbPure™ Antibody Purification Systems.

Benefits include:

• Easy-to-use
• Only takes 10 minutes!
• A centrifuge is the only equipment needed
• Exchange your buffer and concentrate your antibody if required

The BSA removal buffer is designed to specifically induce antibody precipitation. Other proteins such as BSA are unaffected and remain in solution, allowing them to be easily removed. The precipitated antibodies can then be dissolved in the supplied ‘conjugation friendly’ buffer.

Schematic procedure:

AbPure™ BSA Removal:

How it works:

The BSA removal buffer is designed to specifically induce antibody precipitation. Other proteins such as BSA are unaffected and remain in solution, allowing them to be easily removed. The precipitated antibodies can then be dissolved in the supplied ‘conjugation friendly’ buffer.