HRP Check&Go!

HRP Check&Go! is a quick immunochromatography test that allows you to confirm the successful conjugation of Horseradish Peroxidase (HRP) to your antibody without the need for any specialized or costly equipment.

The key component of the kit is a nitrocellulose membrane containing a Test line of immobilized Protein A and Protein G called a half strip. The HRP-antibody conjugate is run on the Protein A/G Strip. The conjugate binds the Protein A and Protein G concentrated on the Test line.

After the addition of the HRP detection solution, a visible line on the strip appears.

Features and benefits:

• Quickly check the success of your conjugation (only 25 minutes!)
• Easy to use
• Sensitive – range of detection is between 0.5ng/ml-10ng/ml
• No costly equipment required
• Compatible with antibody conjugates generated using our Lightning-Link® HRP antibody labeling kits, or any other HRP conjugation technologies available commercially
• Requires only small volumes (40ul of diluted conjugate)
• Unique to Innova Biosciences

Figure 1. Goat anti-rabbit IgG was conjugated with Lightning-Link® HRP and run at different concentrations on Protein A/G strips. After the addition of HRP Detection Solution, a blue signal developed at the T-line. The range of detection to obtain a clear, strong result is from 0.5ng/ml to 10ng/ml of antibody conjugate.

Figure 2. IgG antibodies from mouse, rabbit and goat were conjugated with Lightning-Link® HRP and run at different concentrations on Protein A/G strips. After the addition of HRP Detection Solution, a blue signal developed at the T-line. There is a strong visible signal for the IgG of all three species within the suggested range (0.5ng/ml to 10ng/ml of antibody-conjugate).